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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
07/11/2018 |
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Actualizado : |
07/11/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
CASAL, A.; GARCÍA-ROCHE, M.; NAVAJAS, E.; CASSINA, A.; CARRIQUIRY, M. |
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Afiliación : |
ALBERTO CASAL, Universidad de la República (UdelaR)/ Facultad de Agronomía; MERCEDES GARCÍA ROCHE, Universidad de la República (UdelaR)/ Facultad de Agronomía; Center for Free Radical and Biomedical Research (CEINBIO), Universidad de la República (UdelaR)/ Facultad de Medicina.; ELLY ANA NAVAJAS VALENTINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ADRIANA CASSINA, Center for Free Radical and Biomedical Research (CEINBIO), Universidad de la República (UdelaR)/ Facultad de Medicina.; MARIANA CARRIQUIRY, Universidad de la República (UdelaR)/ Facultad de Agronomía. |
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Título : |
Hepatic mitochondrial function in Hereford steers with divergent residual feed intake phenotypes. |
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Complemento del título : |
Article. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Journal of Animal Science, 29 September 2018, volume 96, Issue 10, pages 4431-4443. |
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ISSN : |
1525-3163 |
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DOI : |
10.1093/jas/sky285 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 01 March 2018 // Accepted: 11 July 2018 // Published: 19 July 2018. |
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Contenido : |
ABSTRACT.
Variations in phenotypic expression of feed efficiency could be associated with differences or inefficiencies in mitochondria function due to its impact on energy expenditure. The aim of this study was to determine hepatic mitochondrial density and function in terms of respiration, gene and protein expression, and enzyme activity of mitochondrial respiratory complex proteins, in steers of divergent residual feed intake (RFI) phenotypes. Hereford steers (n = 111 and n = 122 for year 1 and 2, respectively) were evaluated in postweaning 70 d standard test for RFI. Forty-six steers exhibiting the greatest (n = 9 and 16 for year 1 and 2; high-RFI) and the lowest (n = 9 and 12 for year 1 and 2; low-RFI) RFI values were selected for this study. After the test, steers were managed together until slaughter under grazing conditions until they reached the slaughter body weight. At slaughter, hepatic samples (biopsies) were obtained. Tissue respiration was evaluated using high-resolution respirometry methods. Data were analyzed using a mixed model that included RFI group as fixed effect and slaughter date and year as a random effect using PROC MIXED of SAS. RFI and dry matter intake were different (P < 0.001) between low and high-RFI groups of year 1 and year 2. Basal respiration and maximum respiratory rate were greater (P ? 0.04) for low than high-RFI steers when complex II substrates (succinate) were supplied. However, when Complex I substrates (glutamate/malate) were used maximum respiratory capacity tended to be greater (P < 0.09) for low vs. high-RFI steers. Low-RFI steers presented greater mitochondria density markers (greater (P < 0.05) citrate synthase (CS) activity and tended (P ? 0.08) to have greater CS mRNA and mtDNA:nDNA ratio) than high-RFI steers. Hepatic expression SDHA, UQCRC1, and CYC1 mRNA was greater (P ? 0.02) and expression of NDUFA4, NDUFA13, SDHD, UQCRH, and ATP5E mRNA tended (P ? 0.10) to be greater in low than high-RFI steers. Hepatic SDHA protein expression tended (P < 0.08) to be greater while succinate dehydrogenase activity was greater (P = 0.04) and NADH dehydrogenase activity was greater (P = 0.03) for low than high-RFI steers. High-efficiency steers (low-RFI) probably had greater efficiency in hepatic nutrient metabolism, which was strongly associated with greater hepatic mitochondrial density and functioning, mainly of mitochondrial complex II.
© The Author(s) 2018. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved.
For permissions, please e-mail: journals.permissions@oup.com. MenosABSTRACT.
Variations in phenotypic expression of feed efficiency could be associated with differences or inefficiencies in mitochondria function due to its impact on energy expenditure. The aim of this study was to determine hepatic mitochondrial density and function in terms of respiration, gene and protein expression, and enzyme activity of mitochondrial respiratory complex proteins, in steers of divergent residual feed intake (RFI) phenotypes. Hereford steers (n = 111 and n = 122 for year 1 and 2, respectively) were evaluated in postweaning 70 d standard test for RFI. Forty-six steers exhibiting the greatest (n = 9 and 16 for year 1 and 2; high-RFI) and the lowest (n = 9 and 12 for year 1 and 2; low-RFI) RFI values were selected for this study. After the test, steers were managed together until slaughter under grazing conditions until they reached the slaughter body weight. At slaughter, hepatic samples (biopsies) were obtained. Tissue respiration was evaluated using high-resolution respirometry methods. Data were analyzed using a mixed model that included RFI group as fixed effect and slaughter date and year as a random effect using PROC MIXED of SAS. RFI and dry matter intake were different (P < 0.001) between low and high-RFI groups of year 1 and year 2. Basal respiration and maximum respiratory rate were greater (P ? 0.04) for low than high-RFI steers when complex II substrates (succinate) were supplied. However, when Complex I substrates (glutamate/malate) were used ... Presentar Todo |
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Palabras claves : |
BEEF CATTLE; FEED EFFICIENCY; LIVER; MITOCHONDRIA; OXYGEN CONSUMPTION. |
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Asunto categoría : |
L02 Alimentación animal |
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Marc : |
LEADER 03483naa a2200265 a 4500
001 1059279
005 2018-11-07
008 2018 bl uuuu u00u1 u #d
022 $a1525-3163
024 7 $a10.1093/jas/sky285$2DOI
100 1 $aCASAL, A.
245 $aHepatic mitochondrial function in Hereford steers with divergent residual feed intake phenotypes.$h[electronic resource]
260 $c2018
500 $aArticle history: Received: 01 March 2018 // Accepted: 11 July 2018 // Published: 19 July 2018.
520 $aABSTRACT. Variations in phenotypic expression of feed efficiency could be associated with differences or inefficiencies in mitochondria function due to its impact on energy expenditure. The aim of this study was to determine hepatic mitochondrial density and function in terms of respiration, gene and protein expression, and enzyme activity of mitochondrial respiratory complex proteins, in steers of divergent residual feed intake (RFI) phenotypes. Hereford steers (n = 111 and n = 122 for year 1 and 2, respectively) were evaluated in postweaning 70 d standard test for RFI. Forty-six steers exhibiting the greatest (n = 9 and 16 for year 1 and 2; high-RFI) and the lowest (n = 9 and 12 for year 1 and 2; low-RFI) RFI values were selected for this study. After the test, steers were managed together until slaughter under grazing conditions until they reached the slaughter body weight. At slaughter, hepatic samples (biopsies) were obtained. Tissue respiration was evaluated using high-resolution respirometry methods. Data were analyzed using a mixed model that included RFI group as fixed effect and slaughter date and year as a random effect using PROC MIXED of SAS. RFI and dry matter intake were different (P < 0.001) between low and high-RFI groups of year 1 and year 2. Basal respiration and maximum respiratory rate were greater (P ? 0.04) for low than high-RFI steers when complex II substrates (succinate) were supplied. However, when Complex I substrates (glutamate/malate) were used maximum respiratory capacity tended to be greater (P < 0.09) for low vs. high-RFI steers. Low-RFI steers presented greater mitochondria density markers (greater (P < 0.05) citrate synthase (CS) activity and tended (P ? 0.08) to have greater CS mRNA and mtDNA:nDNA ratio) than high-RFI steers. Hepatic expression SDHA, UQCRC1, and CYC1 mRNA was greater (P ? 0.02) and expression of NDUFA4, NDUFA13, SDHD, UQCRH, and ATP5E mRNA tended (P ? 0.10) to be greater in low than high-RFI steers. Hepatic SDHA protein expression tended (P < 0.08) to be greater while succinate dehydrogenase activity was greater (P = 0.04) and NADH dehydrogenase activity was greater (P = 0.03) for low than high-RFI steers. High-efficiency steers (low-RFI) probably had greater efficiency in hepatic nutrient metabolism, which was strongly associated with greater hepatic mitochondrial density and functioning, mainly of mitochondrial complex II. © The Author(s) 2018. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
653 $aBEEF CATTLE
653 $aFEED EFFICIENCY
653 $aLIVER
653 $aMITOCHONDRIA
653 $aOXYGEN CONSUMPTION
700 1 $aGARCÍA-ROCHE, M.
700 1 $aNAVAJAS, E.
700 1 $aCASSINA, A.
700 1 $aCARRIQUIRY, M.
773 $tJournal of Animal Science, 29 September 2018, volume 96, Issue 10, pages 4431-4443.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
27/05/2019 |
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Actualizado : |
07/06/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Nacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
SCHOLZ, R.; PEREYRA, S.; GARCIA, R.; GERMAN, S. |
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Afiliación : |
RUTH FABIOLA SCHOLZ DRODOWSKI, Instituto Paraguayo de Tecnología Agraria (IPTA). Capitán Miranda Itapúa Ruta 6 km 16. Paraguay.; SILVIA ANTONIA PEREYRA CORREA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RICHARD ANSELMO GARCIA USUCA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SILVIA ELISA GERMAN FAEDO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Identificación de razas de roya de la hoja del trigo presentes en Uruguay durante 2011-2012. [Identification of Wheat Leaf Rust Races Present in Uruguay during 2011-2012]. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Agrociencia Uruguay, 2019, 23(1):1-9. |
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ISSN : |
1510 0839- ISSN electrónico 2301-1548. |
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DOI : |
10.31285/AGRO.23.1.10 |
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Idioma : |
Español |
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Notas : |
Article history: Recibido: 06-12-2017// Aceptado: 11-09-2018. |
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Contenido : |
Resumen: La roya de la hoja de trigo (RH), causada por Puccinia triticina Ericks, es una de las enfermedades del cultivo más prevalentes en Uruguay y otros países del Cono Sur. En epidemias severas se han estimado pérdidas de rendimiento de más de 50 % y pueden ser necesarias dos o más aplicaciones de fungicidas para controlar la enfermedad en cultivares susceptibles. Los estudios de la variabilidad del patógeno son importantes para determinar su rango de virulencia, para inferir el mecanismo de su variación, determinar el origen y dispersión de los patotipos y confirmar la presencia de nuevas razas inferidas por el cambio de comportamiento del hospedero. El objetivo de esta investigación fue estudiar la diversidad en la población de P. triticina en Uruguay durante 2011 y 2012. Se estudiaron un total de 363 muestras de RH recolectadas en diferentes momentos de distintos cultivares y localidades de la región del cultivo. La prevalencia de las 22 razas identificadas (de mayor a menor frecuencia MFP; TDT-10,20; MDP; MDT-10,20; TFT-10,20; MFP-20; MFP-10,20; MDP-20; DBB-10,20; MKD-10; TPR-20,39; MFT-10,20; MDP-10,20; MDR-10,20; MFT; MDT; MFP-10; MHJ-10; MJD10; TMD-10,20; MCP; MFR-10,20), se relacionó a su virulencia sobre los cultivares utilizados por los agricultores. La presencia o frecuencia de las razas varió entre años, zonas y momento de recolección. Se reportan por primera vez las razas DBB-10,20, MHJ-10, MJD-10, MKD-10, TMD-10,20 y TPR-20,30, no previamente detectadas en Uruguay. Este estudio permitió confirmar que la población del patógeno presente en Uruguay es diversa y continúa evolucionando.
Summary: Leaf rust (LR), caused by Puccinia triticina Ericks, is one of the most prevalent wheat diseases in Uruguay and other countries of the Southern Cone. The economic importance of the disease is clear considering that, in severe epidemics, grain yield losses of more than 50 % have been determined and that two or more fungicide applications are necessary to control the disease in susceptible cultivars. Studies on the pathogen variability are important to determine its range of virulence, infer the mechanism of its variation, determine the origin and spread of pathotypes, complement the knowledge of the resistance of commercial cultivars, confirm the presence of new races inferred by change of the host reaction, and identify races important for breeding and specific studies. The objective of this research was to study the diversity in the P. triticina population in Uruguay during 2011 and 2012. A total of 363 LR samples collected at different times from different cultivars and locations of the crop area were studied. The prevalence of 22 identified races (higher to lower frequency: MFP; TDT-10,20; MDP; MDT-10,20; TFT-10,20; MFP-20; MFP-10,20; MDP-20; DBB-10,20; MKD-10; TPR-20,39; MFT-10,20; MDP-10,20; MDR-10,20; MFT; MDT; MFP-10; MHJ-10; MJD-10; TMD10,20; MCP; MFR-10,20), was related to the virulence on cultivars used by farmers. The presence or frequency of the races varied among years, zones and time of collection. Two races not epreviously reported were detected. This study confirmed that the pathogen population present in Uruguay is diverse and continues to evolve. MenosResumen: La roya de la hoja de trigo (RH), causada por Puccinia triticina Ericks, es una de las enfermedades del cultivo más prevalentes en Uruguay y otros países del Cono Sur. En epidemias severas se han estimado pérdidas de rendimiento de más de 50 % y pueden ser necesarias dos o más aplicaciones de fungicidas para controlar la enfermedad en cultivares susceptibles. Los estudios de la variabilidad del patógeno son importantes para determinar su rango de virulencia, para inferir el mecanismo de su variación, determinar el origen y dispersión de los patotipos y confirmar la presencia de nuevas razas inferidas por el cambio de comportamiento del hospedero. El objetivo de esta investigación fue estudiar la diversidad en la población de P. triticina en Uruguay durante 2011 y 2012. Se estudiaron un total de 363 muestras de RH recolectadas en diferentes momentos de distintos cultivares y localidades de la región del cultivo. La prevalencia de las 22 razas identificadas (de mayor a menor frecuencia MFP; TDT-10,20; MDP; MDT-10,20; TFT-10,20; MFP-20; MFP-10,20; MDP-20; DBB-10,20; MKD-10; TPR-20,39; MFT-10,20; MDP-10,20; MDR-10,20; MFT; MDT; MFP-10; MHJ-10; MJD10; TMD-10,20; MCP; MFR-10,20), se relacionó a su virulencia sobre los cultivares utilizados por los agricultores. La presencia o frecuencia de las razas varió entre años, zonas y momento de recolección. Se reportan por primera vez las razas DBB-10,20, MHJ-10, MJD-10, MKD-10, TMD-10,20 y TPR-20,30, no previamente detectadas en ... Presentar Todo |
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Palabras claves : |
PATHOGEN POPULATION; POBLACIÓN DE PATÓGENO; PUCCINIA TRITICINA; RACES; RAZAS. |
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Thesagro : |
TRIGO; TRITICUM AESTIVUM. |
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Asunto categoría : |
F01 Cultivo |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/12699/1/Agrociencia-Uruguay-v.23.n.1.p.1-9-2019-Pereyra-et-al.pdf
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Marc : |
LEADER 04169naa a2200277 a 4500
001 1059783
005 2019-06-07
008 2019 bl uuuu u00u1 u #d
022 $a1510 0839- ISSN electrónico 2301-1548.
024 7 $a10.31285/AGRO.23.1.10$2DOI
100 1 $aSCHOLZ, R.
245 $aIdentificación de razas de roya de la hoja del trigo presentes en Uruguay durante 2011-2012. [Identification of Wheat Leaf Rust Races Present in Uruguay during 2011-2012].$h[electronic resource]
260 $c2019
500 $aArticle history: Recibido: 06-12-2017// Aceptado: 11-09-2018.
520 $aResumen: La roya de la hoja de trigo (RH), causada por Puccinia triticina Ericks, es una de las enfermedades del cultivo más prevalentes en Uruguay y otros países del Cono Sur. En epidemias severas se han estimado pérdidas de rendimiento de más de 50 % y pueden ser necesarias dos o más aplicaciones de fungicidas para controlar la enfermedad en cultivares susceptibles. Los estudios de la variabilidad del patógeno son importantes para determinar su rango de virulencia, para inferir el mecanismo de su variación, determinar el origen y dispersión de los patotipos y confirmar la presencia de nuevas razas inferidas por el cambio de comportamiento del hospedero. El objetivo de esta investigación fue estudiar la diversidad en la población de P. triticina en Uruguay durante 2011 y 2012. Se estudiaron un total de 363 muestras de RH recolectadas en diferentes momentos de distintos cultivares y localidades de la región del cultivo. La prevalencia de las 22 razas identificadas (de mayor a menor frecuencia MFP; TDT-10,20; MDP; MDT-10,20; TFT-10,20; MFP-20; MFP-10,20; MDP-20; DBB-10,20; MKD-10; TPR-20,39; MFT-10,20; MDP-10,20; MDR-10,20; MFT; MDT; MFP-10; MHJ-10; MJD10; TMD-10,20; MCP; MFR-10,20), se relacionó a su virulencia sobre los cultivares utilizados por los agricultores. La presencia o frecuencia de las razas varió entre años, zonas y momento de recolección. Se reportan por primera vez las razas DBB-10,20, MHJ-10, MJD-10, MKD-10, TMD-10,20 y TPR-20,30, no previamente detectadas en Uruguay. Este estudio permitió confirmar que la población del patógeno presente en Uruguay es diversa y continúa evolucionando. Summary: Leaf rust (LR), caused by Puccinia triticina Ericks, is one of the most prevalent wheat diseases in Uruguay and other countries of the Southern Cone. The economic importance of the disease is clear considering that, in severe epidemics, grain yield losses of more than 50 % have been determined and that two or more fungicide applications are necessary to control the disease in susceptible cultivars. Studies on the pathogen variability are important to determine its range of virulence, infer the mechanism of its variation, determine the origin and spread of pathotypes, complement the knowledge of the resistance of commercial cultivars, confirm the presence of new races inferred by change of the host reaction, and identify races important for breeding and specific studies. The objective of this research was to study the diversity in the P. triticina population in Uruguay during 2011 and 2012. A total of 363 LR samples collected at different times from different cultivars and locations of the crop area were studied. The prevalence of 22 identified races (higher to lower frequency: MFP; TDT-10,20; MDP; MDT-10,20; TFT-10,20; MFP-20; MFP-10,20; MDP-20; DBB-10,20; MKD-10; TPR-20,39; MFT-10,20; MDP-10,20; MDR-10,20; MFT; MDT; MFP-10; MHJ-10; MJD-10; TMD10,20; MCP; MFR-10,20), was related to the virulence on cultivars used by farmers. The presence or frequency of the races varied among years, zones and time of collection. Two races not epreviously reported were detected. This study confirmed that the pathogen population present in Uruguay is diverse and continues to evolve.
650 $aTRIGO
650 $aTRITICUM AESTIVUM
653 $aPATHOGEN POPULATION
653 $aPOBLACIÓN DE PATÓGENO
653 $aPUCCINIA TRITICINA
653 $aRACES
653 $aRAZAS
700 1 $aPEREYRA, S.
700 1 $aGARCIA, R.
700 1 $aGERMAN, S.
773 $tAgrociencia Uruguay, 2019, 23(1):1-9.
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